General Instructions for Using HR SEM Facility

• For HR-SEM analysis, Maximum 3 samples (5 images with EDS per samples) for 1 slot for all Internal and External Users.
• Each HRSEM slot allows a maximum of three (3) samples.
• Each HRSEM slot is 1 hour duration.
• In the absence of users and postal samples and Powder samples, the extra service charges are applicable. Refer the usage charges section.
• Samples for HR-SEM measurements should be completely dry, vacuum & e-beam compatible.
• Powder samples cannot be allowed to image directly. Either make them into pellets or disperse in suitable solvent and coat on silicon or any conductive substrate.
• Data or the images will be transferred to CD/DVD only. Please bring your CD/DVD for the same.
• Timing of the slots should be strictly followed. It includes the sample loading, imaging and data transfer etc.
• Only one researcher/research scholar will be allowed to join the technician inside the SEM room during the SEM imaging from the booked user’s group.
• Before giving the sample for HRSEM analysis, it is suggested that each sample is characterized by any other techniques such as XRD or AFM etc. Proper justification needs to be given for doing the HR-SEM for each sample.
• Magnetic samples can be analyzed by HRSEM, but only in Opti-mode. Magnetic samples should be loaded separately.
• One can request the slot only along with the fee payment details & after uploading the clear proof of the payment (DD or receipt for online transfer with UTR number/IMPS number) in the on-line portal the within 24 hours. The slot will be cancelled if the user will not upload the Payment Prof in the on-line portal the within 24 hours from the time and date of booking requested. (not for slot date)
• Users must upload the payment proof at the time of booking their slot.
• The payment proof must be submitted within 30 minutes of booking.
• If the payment proof is not uploaded within this time, the booking will be automatically cancelled.
• All HR SEM slots are requested only through this online portal well in advance. Your requests approval/rejection will be emailed to you well in advance to the slot date.
• If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance (at least 3 days prior to your booking date.
• In case of slots cancelled within three days of your booking date or absent for your booked slots, the amount paid will be assumed as usage charges. The amount paid will not be refunded or allowed to use for any other SCIF facilities.
• If a service was not availed, i.e., cancelled slots with the approval of admin, the payment made can be adjusted towards an upcoming similar service. Refund of fee will NOT be entertained
• Imaging of outside samples by mentioning as internal samples is treated as the misuse of the machine, and the user groups will be banned from the booking process. In case of collaborative research, the samples of research part done by researcher’s of SRM Group is only considered as internal samples.
• Please bring relevant supporting data/literature to help the technician to know more about your sample & explain him about the expected results.
• Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users.

Non-Conductive / Biological Samples

• The samples should be sputter coated with a metal such as gold/chromium to get high quality and high-resolution images. In case of biological/wet samples, the samples should be dried in a critical point drier (CPD).
• Please discuss with operator in advance while imaging biological/wet/sensitive samples - include more details & justification in your slot request. Note that gold coating and use of CPD will be charged extra. Samples requiring sputter coating or critical point point drying need to be submitted one day in advance.
• The amount paid should match the request made including the additional charges for sputter coating, CPD, copper grids (only for STEM Mode) and sample preparation charges, if any.

General Instructions

• Users are required to report at least 10 minutes before their scheduled slot.
• A delay of more than 5 minutes may result in cancellation of the slot without any refund.
• XRD & HRSEM slots are also available on Saturdays.

1) Prior request from the faculty user with necessary details about the sample is required.
2) Please wear powder free gloves and use nuclease free tips before loading nucleic acid samples onto the Nano Quant Plate. Clean the plate carefully after every use.
3) Please make the slot request only through this online portal well in advance.
4) Within two days you will get your request approved and email will be sent.
5) If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance.
6) Only 45 minutes (one slot) will be spent for one booking including cleaning, data transfer etc. If more time is required or more samples to be done-more slots can be booked.
7) Maximum two researchers, faculty/research scholar will be allowed to join the technician inside the instrument room during sample measurement.
8) Please bring relevant supporting data/literature to help the technician to know more about your sample & explain about expected results.
9) Data or the images will be transferred to CD only. Please bring your CD for the same.
10) You may acknowledge the facility if you proceed for any journal publications.

1) You may send a request to avail the facility with necessary detains of the samples
2) You may give slot request only through online at least prior a week
3) You may receive an approval mail within 2-3 days
4) You may please cancel the slot(s) online if booked slot(s) cannot be used
5) You will be given half a day per slot. You may the book the second slot too if needed more time
6) A maximum of only 2 persons will be allowed to use the facility per slot
7) You may bring your own consumables to run your experiment. No consumables will be provided from the facility. You may be allowed to use only the facility free of cost
8) You may carry out the sample preparation in the working area provided
9) You may bring supporting documents/literatures for sample preparation
10) You may clean the working platform before you would start your experiment
11) You must bring a CD of your own to take out the results from the facility. Results may be deleted once your slot is completed. The facility is not responsible for the any of your results once you step out of the facility
12) You may acknowledge the facility if you proceed for any journal publications.

1) You may send a request to avail the facility with necessary detains of the samples.
2) You may give slot request only through online at least prior a week regarding the use.
3) You may receive an approval mail within 2-3 days.
4) You may please cancel the slot(s) online if booked slot(s) cannot be used.
5) You will be given 30 minutes per slot. You may the book the second slot too if needed more time.
6) A maximum of only 2 persons will be allowed to use the facility per slot.
7) You may bring your own consumables needed. No consumables will be provided from the facility. You may be allowed to use only the facility free of cost.
8) You may carry out the sample preparation in the working area provided.
9) You may bring supporting documents/literatures for sample preparation.
10) You may clean the working platform before you would start your experiment.
11) You must bring a CD of your own to take out the results from the facility. Results may be deleted once your slot is completed. The facility is not responsible for the any of your results once you step out of the facility.
12) You may acknowledge the facility if you proceed for any journal publications.

1) Prior request from the faculty user with necessary details about the sample is required.
2) Before giving the sample for TOC analysis, it is suggested that each sample is studied by UV analysis.
3) Please make the slot request only through this online portal well in advance.
4) On booking the slots, the fee has to be paid either through demand draft (DD) in favor of “SRMIST Consultancy” payable at Chennai or through online transfer (NEFT/IMPS).
Within two days you will get your request approved and email will be sent. One can use the slot by submitting the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number) to the TOC operator in the Energy and Environmental Remediation Lab (EERL).
5) If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance.
6) The sample prepared should be minimum 15 mL – 20 mL for the analysis.
7) The sample flow line diameter is 0.5 mm. Therefore, in order to prevent clogging, any particle larger than this size should be eliminated from the sample, for example by means of sedimentation.
8) Once the sample is ready make sure sample is completely filtered, (it should be completely free from catalyst).
9) The sample must be stored and sealed properly in order to prevent the exposure to carbon content.
10) Please bring relevant supporting data/literature to help the technician to know more about your sample & explain him about the expected results.
11) Data will be transferred to CD only. Please bring your CD for the same.
12) If a service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund will NOT be entertained.
13) Any journal publications/presentations/proceedings etc. arising out of the research facility must be acknowledged by the users.

1) Prior request from the faculty user with necessary details about the sample is required.
2) Before giving the sample for HRMS analysis, it is suggested that each sample is characterized by any other techniques such as GCMS, and 1HNMR etc. Proper justification need to be given for doing the HRMS for each sample.
3) Samples for HRMS measurements should be organic molecules, metal complexes soluble in Water, Acetonitrile or Methanol.
4) Please make the slot request only through this online portal well in advance.
5) Please make the fee payment as per the norms, and upload the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number) online. Within two days you will get your request approved and email will be sent.
6) If a service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund of fee will NOT be entertained.
7) If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance.
8) Suitably prepared samples should be submitted in advance to the slot time at the HRMS facility.
9) Only up to 60 minutes (one slot) will be spent per one sample including the sample loading, data transfer etc. If more time is required or more samples to be done-more slots can be booked.
10) Maximum two researchers, faculty/research scholar will be allowed to join the technician inside the HRMS room during the sample observation.
11) Please bring relevant supporting data/literature to help the technician to know more about your sample & explain him about the expected results.
12) Data will be transferred to CD only. Please bring your CD for the same.
13) Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users. .

1)Prior request from the faculty user with necessary details about the operation is required.
2) Mini micro pulse Lidar Operation Procedure:
• Make sure that power supply and air conditioner are working properly.
• Maintain the room temperature below 20˚C
• Switch on the main power for Lidar and laptop
• Open the lid of laser
• Switch on the power of Lidar and Laptop
• Switch on the laser power key.
• In Laptop open sigma MPL software and click data collecting option.
• Once the data collection started check the laser energy and temperature which has to maintain that laser energy above 3 μj and temperature should below 25 ˚C.
3)Please make the slot request only through this online portal well in advance
4) Once it is approved, please make the fee payment as per the norms. One can use the slot by submitting the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number).
5)If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance.
6)The specific duration of the lidar operation based on the requirement on the regular mode or campaign mode will be considered and booked for 3 days per month. Maximum two researchers, faculty/research scholar will be allowed to join the technician inside the instrument room during Lidar Operation.
7) Data transferred to CD only. Please bring your CD for the same.
8)If a service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund of fee will NOT be entertained.
9)Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users. .

1) Prior request from the user is required.
2)There is no fee required for the use of microscope. The user has to bring CD for copying the data.

1) Prior request from the user is required.
2) Quartz tubes with diameter 10 mm or less can be vacuum sealed.
3) Quartz tubes can be availed at an additional cost.
4) If service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund of fee will NOT be entertained.

1)Prior request from the user is required.
2)If service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund of fee will NOT be entertained.

1) Prior request from the faculty user with necessary details is required.
2) Before requesting for the PL measurement, it is required that each sample is characterized by basic characterization techniques such as XRD, UV-visible spectroscopy.
3) Prior to online request, user need to 1) find out the necessary parameters such as excitation wavelength, emission range etc. based on the literature or inputs from other experiments technique such as UV-visible spectroscopy. 2) Estimate properly the number of recordings needed and the slot duration required.
4) Please make the slot request only through this online portal well in advance. If a measurement other than recording of the regular room temperature PL spectra is required, before requesting online, please contact us.
5) Duration: Please estimate as per the number of recordings required. Generally, if a sample loading was followed by one measurement, we can do 3-4 samples in an hour. If the measurements are only by quickly changing some measurement parameter after loading one sample, then about 10 recordings may be possible with one hour time. Measurements other than regular PL will require more time. Please contact PL lab.
6) The requested number of measurements/recordings possible in the duration of the slot booked only will be done. Please make a correct estimate of the required number of recordings and accordingly the slot time required. Change in the slot duration will not be entertained.
7) Consumables if necessary for one’s experiment such as solvents, standard samples, liquid nitrogen etc, should be arranged by the user.
8) Please make the fee payment as per the norms. Requests will be approved only after the user uploads the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number). Within a week or so, you can expect an update from us about the measurement date & timings.
9) If a request was made and you are not in a position to do the measurement on the date & time offered, please cancel the request through this online portal immediately.
10) Users should plan the sample preparation accordingly if any and bring the prepared sample to the PL lab well before the said time.
Sample requirements:
a) Liquid samples – clear and homogenous solution, minimum 2.5ml, maximum- 4.5ml (concentration- OD 2.)
b) Powder samples- Fine powder, minimum 0.5g required.
c) Films/coated samples- uniform surface, size- 2 cm* 2 cm
11) The operator will help the user to load the prepared samples followed by the requested measurements.
12) At least one faculty or PhD research scholar from the user’s group should be present with the technician during the sample measurement.
13) User can bring relevant supporting data/literature to help the technician to know more about the sample & explain about the expected results.
14) Data will be transferred to CD only. Please bring your CD for the same.
15) If a service was not availed, the payment made can be adjusted towards an upcoming similar service through a fresh request. Refund of fee will NOT be entertained.
16) Any journal publications/presentations/proceedings etc. arising out of the research facility must be acknowledged by the users.

1) Prior request from the faculty user with necessary details is required.
2) Before requesting for the optical profilometer measurement, it is required that each sample is characterized by basic characterization techniques such as UV-visible spectroscopy (opaque sample or sample reflectivity greater than 5% is needed).
3) Please make the slot request only through this online portal well in advance.
4) Duration: Generally, if a sample loading, focusing scanning was followed by only one measurement, we can do 4-5 samples in an hour. Please include the time for analysis as required.
5) Within a week or so, you will get an update from us about the measurement date & timings. Please make the fee payment as per the norms. Slots will be approved only after uploading the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number).
6) If a request was made and you are not in a positon to do the measurement on the provided date & time, please cancel the request through this online portal immediately.
7) The operator will help the user to analyze the sample followed by doing the requested measurements.
8) The requested number of measurements/recordings possible in the duration of the slot booked only will be done. Please make a correct estimate of the required number of recordings and accordingly the slot time required. Change in the slot duration will not be entertained.
9) At least one faculty or Ph. D.research scholar should be present with the technician during the sample measurement.
10) Data will be transferred to CD only. Please bring your CD for the same.
11) If a service was not availed, the payment made can be adjusted towards an upcoming similar service.
12) Any journal publications/presentations/proceedings etc. arising out of the research facility must be acknowledged by the users.

1) Prior request from the faculty user with necessary details is required.
2) Users should plan fabrication of the devices with options for taking proper contacts and bring the prepared sample to the lab well before the said time.
3) Before requesting for the I-V measurement under the solar simulator, it is required that each device is tested for proper contacts and I-V in dark condition.
4) Please make the slot request only through this online portal well in advance.
5) Within a week or so, you will get an update from us about the measurement date & timings. Please make the fee payment as per the norms. Slots will be approved only after uploading the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number).
6) If a request was made and you are not in a positon to do the measurement on the provided date & time, please cancel the request through this online portal immediately.
7) The operator will help the user to connect the devices to the I-V meter followed by doing the requested measurements.
8) The requested number of measurements/recordings will be done only if the slot time allows the same. Please make a correct estimate of the slot time required. Change in the slot duration will not be entertained.
9) At least one faculty or PhD research scholar should be present with the technician during the sample measurement.
10) Data will be transferred to CD only. Please bring your CD for the same.
11) If a service was not availed, the payment made can be adjusted towards an upcoming similar service.
12) Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users.

1) Prior request from the faculty user with necessary details about the sample is required.
2) Before giving the sample for STM analysis, it is suggested that each sample is characterized by any other technique such as XRD, FESEM, AFM, EDS, surface profiling etc. Proper justification need to be given for using the STM facility for each sample.
3) Samples for STM analysis should be completely dry & should be coated on a conductive substrate (e.g. HOPG, Gold coated mica substrate etc). Powder samples (uncoated) cannot be analyzed by STM. Please discuss with us/operator in advance - include more details & justification in your slot request. No samples will be entertained without prior discussion.
4) Please make the slot request only through this online portal well in advance.
5) Within two days you will get your request approved and an Email will be sent. Once it is approved, please make the fee payment as per the norms. One can use the slot by submitting the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number) to the STM operator in the STM lab.
6) If the booked slots cannot be utilized, please cancel it through this online portal well in advance.
7) The prepared samples should be submitted in advance to the slot time at the STM facility.
8) Only 90 minutes (one slot) will be spent per sample including the sample loading, data transfer etc. If more time is required or more samples are to be done, more slots have to be booked.
9) Only one researcher (faculty/research scholar), will be allowed to join the technician inside the STM room during the sample observation.
10) Please bring relevant supporting data/literature to help the technician to know more about your sample & explain him about the expected results.
11) Data or the images will be transferred to CD only. Please bring your CD for the same.
12) If a service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund of fee will NOT be entertained.
13) Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users. .

1) Prior request from the faculty user with necessary details about the sample is required.
2) Before giving the sample for qPCR analysis, it is suggested to bring the following contents,
• FastStart Essential DNA Green Master, 2x concentrated.
• Water, PCR Grade
• Reaction setup requires the addition of template DNA and primers. The mix can be used with different types of DNA (e.g., genomic, cDNA).
• Disposable 96 well plates or 8 tube strips (suitable for LightCycler® 96 Real-Time PCR System)
3) Please make the slot request only through this online portal well in advance.
4) Within two days you will get your request approved and email will be sent. Once it is approved, please make the fee payment as per the norms. One can use the slot by submitting the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number).
5) If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance.
6) Only 2 to 3 hours (one slot) will be spent per. If more time is required or more samples to be done-more slots can be booked.
7) Data or the images will be transferred to CD only. Please bring your CD for the same.
8) If a service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund of fee/return of grids will NOT be entertained.
9) Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users. .

1) Prior request from the faculty user with necessary details about the sample is required.
2) Please make the slot request only through this online portal well in advance.
3) On booking the slots, the fee has to be paid either through demand draft (DD) in favor of “SRMIST Consultancy” payable at Chennai or through online transfer (NEFT/IMPS). Within two days you will get your request approved and email will be sent. One can use the slot by submitting the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number) to the Trace Analyser operator in the Energy and Environmental Remediation Lab (EERL).
4) If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance.
5) The sample prepared should be in a liquid state.
6) Please bring relevant supporting data/literature to help the technician to know more about your sample & explain him about the expected results.
7) Data will be transferred to CD only. Please bring your CD for the same.
8) If a service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund will NOT be entertained.
9) Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users.

1) Prior request from the faculty user with necessary details about the sample is required.
2) Before giving the sample for HPLC analysis, it is suggested that each sample is studied by TLC analysis and the right solvents are to be studied.
3) Please make the slot request only through this online portal well in advance by paying the necessary fee and uploading the payment proof as per the guidelines.
4) Within two days you will get your request approved and email will be sent. One can use the slot by submitting the proof of the fee payment (DD or receipt for online transfer with UTR number/IMPS number) to the HPLC operator in the Energy and Environmental Remediation Lab (EERL).
5) If the booked slots cannot be utilized, please cancel the slots blocked through this online portal well in advance.
6) The sample prepared should be minimum 2mL for the analysis.
7) The users must bring with them the required capillary tube.
8) Once the sample is ready make sure sample is completely filtered, (it should be completely free from catalyst) or dissolved in the suitable solvent.
9) Please bring relevant supporting data/literature to help the technician to know more about your sample & explain him about the expected results
10) Data will be transferred to CD only. Please bring your CD for the same.
11) If a service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund will NOT be entertained.
12) Any journal publications/presentations/proceedings etc arising out of the research facility must be acknowledged by the users.

1) Prior request from the user is required.
2) If service was not availed, the payment made can be adjusted towards an upcoming similar service. Refund of fee will not be entertained.